Adipose Tissue Protocols by Gema Frühbeck (auth.), Kaiping Yang (eds.)

By Gema Frühbeck (auth.), Kaiping Yang (eds.)

Since the ebook of the 1st version, the recommendations and experimental techniques hired within the learn of adipose tissue have gone through radical enlargement and revision. In Adipose Tissue Protocols: moment Edition, a number of up-to-date chapters are mixed with many new contributions from the world over famous specialists masking either traditional and cutting-edge methodologies in addition to entire animal, mobile and molecular techniques that have emerged in or been prolonged to adipose tissue examine over the last few years. As a quantity within the hugely sucessful Methods in Molecular Biology™ sequence, each one bankruptcy contains an creation to its material, an inventory of worthy fabrics, step by step, without problems reproducible laboratory protocols, and a Notes part, which gives helpful counsel and tips to guard opposed to power mishaps and pitfalls.

Cutting-edge and entire, Adipose Tissue Protocols: moment Edition is an important guide for beginners to this burgeoning zone in addition to a worthwhile addition to the laboratories and workplaces of more matured researchers.

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Brown fat appears as a butterfly between shoulders (C,D) Front and side views, respectively, of inguinal fat 32 L. Casteilla et al. 6. Rub the fat pad with a paper tissue to discard the white part just above the IBAT and then carefully dissect the pad. 7. A binocular microscope can be used but, with some practice, this is not necessary; or remove the fat pad, and afterwards carefully dissect the butterfly of brown fat. In all cases, care must be taken to avoid the muscle closely associated with the brown fat.

J Clin Endocrinol Metab 91:4753–4761 Chapter 2 Choosing an Adipose Tissue Depot for Sampling Factors in Selection and Depot Specificity Louis Casteilla, Luc Pénicaud, Béatrice Cousin, and Denis Calise Summary The importance and the role of adipose tissues are now largely expanded not only because the very high occurrence of obesity but also because the emerging view that adipose tissue could be a reservoir of therapeutic cells. A critical examination of the adipose tissue features according to their location shows that sampling is not as easy as previously thought and needs special attention to heterogeneity and differences.

Nature 444:860–867 80. Tilg H, Moschen AR (2006) Adipocytokines: mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol 6:772–783 81. Lumeng CN, Bodzin JL, Saltiel AR (2007) Obesity induces a phenotypic switch in adipose tissue macrophage polarization. J Clin Invest 117:175–184 82. Lumeng CN, Deyoung SM, Bodzin JL, Saltiel AR (2007) Increased inflammatory properties of adipose tissue macrophages recruited during diet-induced obesity. Diabetes 56:16–23 83. Choudhury J, Sanyal AJ (2004) Clinical aspects of fatty liver disease.

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