By J.A. Callow, H.W. Woolhouse (Eds.)
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Extra resources for Advances in Botanical Research, Vol. 11
On optical benches, so that the specimen can be observed as required while allowing adequate access of the incident and scattered laser beams. , Mishina et a / . , 1975). B . STANDARD TEST SYSTEMS Before embarking upon the examination of biological specimens, it is advisable to study a few well-defined physical systems in which only a limited number of specific motions are occurring. Typical test systems are described below, some of the results presented being derived from the testing of our laser Doppler microscope.
2. Basic Laser Doppler Microscope Our present laser Doppler microscope has evolved from the design described previously (Earnshaw and Steer, 1979a,b). It is based on a Zeiss Universal microscope fitted with an epiillumination objective carrier (Fig. 11). The standard microscope phototube is mounted in place of the binoculars, so that the specimen may be viewed through the eyepiece and photographed as required. A viewing telescope is mounted in an adjustable vertical tube, so that the back focal plane of the objective can be focused onto the plane of a pinhole aperture Kl P.
This pinhole is mounted on the front of the photomultiplier detector tube and the whole assembly bolted to a micrometer-driven x-y translation stage, so that the aperture can be accurately and reproducibly located at any part of the back focal plane. This arrangement allows detection of light scattered at an angle to the vertical axis, and so avoids direct reflections of the unscattered incident beam. A He-Ne laser is mounted on an optical bench; both ends of the laser can be moved vertically and horizontally to permit the light beam to be centered on and made parallel to the optical axis of the microscope.