Advances in Microbial Physiology, Vol. 20 by A. H. & Morris, J. Gareth [eds.] Rose

By A. H. & Morris, J. Gareth [eds.] Rose

This quantity in a research-level sequence covers assorted elements of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial mobile partitions. it's meant to be of use to microbiologists, biochemists and biotechnologists. different similar works during this sequence are volumes 29, 30 and 31.

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1976). In A. niduluns, these novel RNAs appear to be synthesized both in light and darkness but in the light they, like mRNA, turn over rapidly and can only be demonstrated during very short labelling periods. In darkness they appear completely stable. Novel RNAs accumulated in darkened cells are rapidly (t3 6 min) and apparently completely degraded when cells are re-illuminated. Degradation after re-illumination is retarded (t3 14 min) by the addition of chloramphenicol. It is not, however, darkness per se which is required for the accumulation of novel RNAs.

G. double strand scission or “post-replicational gaps” opposite unexcised pyrimidine dimers), which might be expected in brutally irradiated cells. Shestakov et al. (1976) have presented evidence for recombinational repair in A . nidulans. V. Cyanobacterial Ribonucleic Acids: Synthesis, Processing and Sequence Characterization A. R N A P O L Y M E R A S E RNA polymerase activity was first demonstrated in A . nidulans more than ten years ago (Capesius and Richter, 1967; von der Helm and Zillig, 1967) and this enzyme is now relatively well characterized.

Such methods have also allowed the identification ofCCC (presumably plasmid) DNAs ofas yet unknown (cryptic) genetic composition in many prokaryotes including, quite recently, numerous cyanobacteria. Asato and Ginoza (1972, 1973) demonstrated that a fraction of Anacystis nidulans DNA was separable from the bulk by ethidium-bromide cesium-chloride gradient centrifugation. This fraction was not separated from the main band in gradients lacking ethidium bromide, reannealed rapidly after alkali denaturation, and was deoxyribonuclease-sensitive, all properties expected of a plasmid of GC content s'imilar to that of the chromosome.

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