An Introduction to Microbiology. Pharmaceutical Monographs by W. B. Hugo, J. B. Stenlake

By W. B. Hugo, J. B. Stenlake

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E09 then, is the value of the electrode potential at 50 per cent oxidation, or 50 per cent reduction, and is called the standard electrode potential for the system; it is a measure of the proneness of a system to yield electrons, that is to undergo oxidation just as pH is a measure of the proneness of an acid to yield hydrogen ions.

It should 39 AN INTRODUCTION TO MICROBIOLOGY be realised that in this stage every cell is not dividing, as it is also not strictly true to say that, in the stationary phase, no cell divides. Nevertheless, the general pattern of a lag and log phase is characteristic. 3. A stationary phase. Following upon the phase of active growth, there follows a phase when again little or no growth occurs; this is referred to as the stationary phase. The most obvious explanation of this situation is that it comes about because of exhaustion of essential food materials by the multiplying cells; a variety of experiments has suggested that other factors may be involved, such as the achievement of a toxic pH or the accumulation of a toxic metabolite.

Coli requiring factors which may be called b, c and d, and secondly a strain requiring e and/; thus the first strain differs from the parent nonexacting strain in lacking the ability, through gene deficiency, of synthesising b, c and d and the same argument applies to the second strain with respect for e and / . The two deficient strains were grown together in a medium supplied with the factors è, c, d9 e a n d / ; thus all grew. After growth, some of the cells were transferred to the minimal medium, when about one in each million cells transferred could grow.

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