By Alvin Fox, James Gilbart, Stephen L. Morgan (auth.), Alvin Fox, Stephen L. Morgan, Lennart Larsson, Göran Odham (eds.)
The First foreign Symposium at the Interface among Analytical Chemistry and Microbiology: functions of Chromatography and Mass Spectrometry was once held June 1987 on the collage of South Carolina, Columbia, SC, U.S.A. the aim of the "Interface" assembly was once to forge connections among analytical chemists and microbiologists which are utilizing chromatography and mass spectrometry to unravel universal difficulties. The ambitions have been admirably fulfilled. approximately 100 individuals from seven ecu nations, Japan, and the us participated in listening to twenty-three plenary talks and thirty-six submitted papers and posters. The papers and discussions displayed the breadth and intensity of present learn functions and published destiny instructions. This e-book "Analytical Microbiology equipment: Chromatography and Mass Spectrometry" is loosely in keeping with a few of the displays and discussions on the assembly. each one bankruptcy describes particular method and purposes within the context of the appropriate clinical history. the current publication keeps the subject matter of an prior e-book, "Gas Chromatography/Mass Spectrometry purposes in Microbiology", edited by way of G. Odham, L. Larsson, and P-A. Mardh, released by means of Plenum Press in 1984.
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Additional info for Analytical Microbiology Methods: Chromatography and Mass Spectrometry
Microbiological applications of CC-MS to the analysis of carbohydrates (Chapter 5 and 15), amino acids (Chapter 6), and fatty acids (Chapters 4, 14 and 16) are discussed in this text. Pure standards for microbial components are often not available and qualitative identification of compounds by MS is an important driving force for its use. The mass spectrum of a compound is often sufficient for chemical identification. Even if the mass spectrum cannot be fully interpreted or matched to a library spectrum or standard, characteristic fragmentation patterns and masses often suggest structural features.
111-114 The frequency of the revolution (cyclotron frequency) is inversely proportional to the mass-to-charge ratio of the ion; the radius of the circular trajectory is proportional to ion velocity and inversely proportional to frequency of revolution. When an rf electric field is applied perpendicular to both the magnetic field and the initial direction of motion of the ion, and when its frequency matches the cyclotron frequency, the ion will absorb energy and begin to spiral (a resonance condition).
The quadrupole ion trap (QIT) has been widely used as a detector for GC because of its compactness: an ionizer assembly is not required. 112 A typical QIT consists of a ring electrode and two end-cap electrodes. An rf voltage is applied to the ring electrode, while the two end-cap electrodes are grounded. sample is introduced through one of the end-cap electrodes and ionized by EI. Under appropriate conditions, all ions whose m/z ratios are greater than a minimum cut-off value (set by the magnitude of the rf voltage) are trapped; smaller ions are ejected through the other end-cap electrode and detected with an electron multiplier.