Nitrogen Metabolism in Plants in the Post-genomic Era by Christine Foyer, Hanma Zhang

By Christine Foyer, Hanma Zhang

Nitrogen Metabolism in vegetation presents a common heritage and review of nitrogen acquisition in crops and offers a entire description of modern advances in our realizing of either nitrogen assimilation and nitrogen fixation utilizing new applied sciences. this significant new e-book covers many vital elements, together with delivery structures, regulatory and signaling mechanisms, plant improvement and senescence, and metabolic cross-talk among nitrogen assimilation and different metabolic pathways. a part of Wiley-Blackwell's hugely acclaimed and winning Annual Plant experiences, this new quantity is an important buy for all plant scientists.

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2002) were able to express the A. thaliana gene in E. coli. The purified asparaginase protein was shown to comprise peptides of approximately 35, 24 and 12 kDa, following SDS-PAGE. The authors proposed that the two smaller peptides were the result of proteolytic cleavage and that the native protein, rather than being a dimer, was in fact an (␣␤)2 -tetramer. Analysis of the substrate specificity of the recombinant A. thaliana protein showed that the enzyme could use a range of ␤-aspartyl peptides as substrates, with ␤-aspartyl-phenylalanine and ␤-aspartyl-alanine having V max values close to that of asparagine.

1999; Restivo, 2004) that code for the ␣- and ␤-subunits, respectively. Masclaux-Daubresse et al. (2002) isolated a GDH1 gene from N. tabacum coding for a ␤-subunit that was 95% sequence identical with the GDHB gene from N. plumbaginifolia. Purnell et al. (2005) isolated two partial cDNA clones for GDH that were 98% c01 BLBK316-Foyer August 25, 2010 16:28 Trim: 234mm×156mm Series: APR Char Count= Nitrogen Assimilation and Its Relevance to Crop Improvement 15 homologous to the N. plumbaginifolia genes GDHA and GDHB, respectively.

2006; Miyashita & Good, 2008), which would be expected if At3g03910 led to the expression of a functional protein. The conclusion is that there is probably only one functional gene for the ␤-subunit in A. thaliana. Genes for GDH have been isolated from Nicotiana species. , 1999; Restivo, 2004) that code for the ␣- and ␤-subunits, respectively. Masclaux-Daubresse et al. (2002) isolated a GDH1 gene from N. tabacum coding for a ␤-subunit that was 95% sequence identical with the GDHB gene from N.

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