By Christine Foyer, Hanma Zhang
Nitrogen Metabolism in vegetation presents a common heritage and review of nitrogen acquisition in crops and offers a entire description of modern advances in our realizing of either nitrogen assimilation and nitrogen fixation utilizing new applied sciences. this significant new e-book covers many vital elements, together with delivery structures, regulatory and signaling mechanisms, plant improvement and senescence, and metabolic cross-talk among nitrogen assimilation and different metabolic pathways. a part of Wiley-Blackwell's hugely acclaimed and winning Annual Plant experiences, this new quantity is an important buy for all plant scientists.
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Extra info for Nitrogen Metabolism in Plants in the Post-genomic Era
2002) were able to express the A. thaliana gene in E. coli. The purified asparaginase protein was shown to comprise peptides of approximately 35, 24 and 12 kDa, following SDS-PAGE. The authors proposed that the two smaller peptides were the result of proteolytic cleavage and that the native protein, rather than being a dimer, was in fact an (␣␤)2 -tetramer. Analysis of the substrate specificity of the recombinant A. thaliana protein showed that the enzyme could use a range of ␤-aspartyl peptides as substrates, with ␤-aspartyl-phenylalanine and ␤-aspartyl-alanine having V max values close to that of asparagine.
1999; Restivo, 2004) that code for the ␣- and ␤-subunits, respectively. Masclaux-Daubresse et al. (2002) isolated a GDH1 gene from N. tabacum coding for a ␤-subunit that was 95% sequence identical with the GDHB gene from N. plumbaginifolia. Purnell et al. (2005) isolated two partial cDNA clones for GDH that were 98% c01 BLBK316-Foyer August 25, 2010 16:28 Trim: 234mm×156mm Series: APR Char Count= Nitrogen Assimilation and Its Relevance to Crop Improvement 15 homologous to the N. plumbaginifolia genes GDHA and GDHB, respectively.
2006; Miyashita & Good, 2008), which would be expected if At3g03910 led to the expression of a functional protein. The conclusion is that there is probably only one functional gene for the ␤-subunit in A. thaliana. Genes for GDH have been isolated from Nicotiana species. , 1999; Restivo, 2004) that code for the ␣- and ␤-subunits, respectively. Masclaux-Daubresse et al. (2002) isolated a GDH1 gene from N. tabacum coding for a ␤-subunit that was 95% sequence identical with the GDHB gene from N.